Figure 2
cDKO mice had hearts with abnormal morphologies and impaired functions. (a) Gross appearance of hearts from Meox2+/+PLCδ1fl/−PLCδ3+/− (Hetero), Meox2cre/+PLCfl/−PLCδ3+/− (PLCδ1KO), Meox2+/+PLCδ1fl/−PLCδ3−/− (PLCδ3KO), and Meox2cre/+PLCδ1fl/−PLCδ3−/− (cDKO) mice at 4, 6, 8, and 12 weeks of age. Scale bar, 2 mm. The boxed areas in the cDKO hearts at 6, 8, and 12 weeks are magnified in the right panels. The arrows indicate white foci. (b) Heart (upper panel) or liver (lower panel) weight-to-tibial length ratios of Meox2+/+PLCδ1fl/−PLCδ3+/− (hetero), Meox2cre/+PLCfl/−PLCδ3+/− (PLCδ1KO), Meox2+/+PLCδ1fl/−PLCδ3 −/− (PLCδ3KO), and Meox2cre/+PLCδ1fl/−PLCδ3−/− (cDKO) mice. Mean+S.E.M. (hetero, n=3; PLCδ1KO, n=6; PLCδ3KO, n=6; and cDKO, n=5). (c) Quantitative data of echocardiographic measurements. Echocardiograms were measured in Meox2+/+PLCδ1fl/−PLCδ3+/− (hetero), Meox2cre/+PLCfl/−PLCδ3+/− (PLCδ1KO), Meox2+/+PLCδ1fl/−PLCδ3−/− (PLCδ3KO), and Meox2cre/+PLCδ1fl/−PLCδ3−/− (cDKO) mice at 4, 6, 8, and 12 weeks of age. Mean±S.E.M. (hetero, n=6; PLCδ1KO, n=6; PLCδ3KO, n=7; and cDKO, n=5 at each time point). EF, ejection fraction; FS, fraction shortening; ESV, end-systolic volume; and EDV, end-diastolic volume. (d) Representative M-mode echocardiograms of Meox2+/+PLCδ1fl/−PLCδ3+/− (hetero), Meox2cre/+PLCfl/−PLCδ3+/− (PLCδ1KO), Meox2+/+PLCδ1fl/−PLCδ3−/− (PLCδ3KO), and Meox2cre/+PLCδ1fl/−PLCδ3−/− (cDKO) mice at 8 weeks of age. (e) mRNA expression or ratios of cardiac failure markers in the hearts of Meox2+/+PLCδ1fl/−PLCδ3+/− (hetero), Meox2cre/+PLCfl/−PLCδ3+/− (PLCδ1KO), Meox2+/+PLCδ1fl/−PLCδ3−/− (PLCδ3KO), and Meox2cre/+PLCδ1fl/−PLCδ3−/− (cDKO) mice at 8 weeks of age. The results are listed in arbitrary units (expression or ratio in hetero=1). Mean+S.E.M. (hetero, n=6; PLCδ1KO, n=3; PLCδ3KO, n=3; and cDKO, n=4). Statistical significance was assessed using Tukey–Kramer’s post hoc test. *P<0.05 and **P<0.01
