Figure 3
cDKO mice showed cardiac fibrosis. (a, b) Hematoxylin and eosin (a) and Masson trichrome (b)-stained sections from the left ventricles of control (Meox2+/+PLCδ1fl/−PLCδ3+/−) and cDKO mice at 4, 6, 8, and 12 weeks of age. The arrows indicate fibrotic areas (blue). Scale bar, 100 μm. (c) mRNA expression of cardiac fibrosis markers in the hearts of cDKO mice at 8 weeks of age. The results are listed in arbitrary units (expression in control mice=1). Mean+S.E.M. (control, n=12; cDKO, n=4). (d) Systolic, diastolic blood pressure of 4- or 6-week-old control and cDKO mice. Mean+S.E.M. (4 weeks, male: control, n=9; cDKO, n=4; 4 weeks, female: control, n=9; cDKO, n=3; 6 weeks, male: control, n=10; cDKO, n=4; 6 weeks, female, control, n=11; and cDKO, n=4). (e) Hematoxylin and eosin-stained sections from left ventricles of control (Meox2cre/+PLCδ1fl/−PLCδ3+/−), and cDKO mice at 12 weeks of age. Scale bar, 50 μm. Quantitative data of myocyte cross-sectional areas are also shown. Mean+S.E.M. 100 cells per section. (Control, n=3 and cDKO, n=3). Statistical significance was assessed using Student’s t-test. *P<0.05 and **P<0.01
