Figure 2
Kinase inhibitor library screening and titration assays of positive hits and analogs in GSCs. (a) Point chart of the viability of two GSCs and two commercial GBM cell lines treated with the kinase inhibitor library each compound at 5 μM for 72 h. Normalized viability of each cell line is plotted as mean±S.D. from three independent experiments. Horizontal dashed lines represent reference lines for 100% (dark green) and 50% (red) viability. (b) Parallel plots of fitted 4PL curves and correspondent hierarchical clustering maps of pEC50 values (see Materials and Methods section for details) derived from titration curves of GSCs treated for 72 h with positive hits from the library screening and (c and d) analog compounds. Fitting was performed on individual dose-response curves obtained from three independent experiments
