Figure 3
From: αNAC inhibition of the FADD-JNK axis plays anti-apoptotic role in multiple cancer cells
FADD mediates the apoptotic effect of αNAC depletion. (a) With or without αNAC depletion, PC3 cells or Hela cells were subjected to colony formation assays (left panel). (b) With or without αNAC depletion, PC3 or PC3-E6 cells were subjected to colony formation assay (left panel). (c) PC3 cells with αNAC and (or) FADD depletion were subjected to colony formation assays (left panel). For a, b and c, all colonies were counted and analyzed (middle panel). When the cells were seeded for colony formation assays, 1 × 106 cells were kept for western blots to confirm the depletion efficiency (right panel). Some cells from experiment c were kept for caspase -3, -8 and -9 activity assays (Figure 3d) or western blots (Figure 3e)
