Figure 4
From: αNAC inhibition of the FADD-JNK axis plays anti-apoptotic role in multiple cancer cells
The MAPK/JNK pathway partially mediates the apoptotic effect of αNAC depletion. (a) PC3 shGFP or shαNAC cells were subjected to Cancer 10-pathway reporter arrays. Luciferase activity represents the pathway activity. For b and c, empty vector, MEKK-KR, or JNK-APF was transfected into PC3 cells. After selection, αNAC was depleted in all three cell lines. Cells were harvested for western blot analysis to investigate the levels of the indicated proteins (b) or were subjected to colony formation assays (c, left panel). Colonies were counted and analyzed (c, right panel)
