Figure 2
From: Sec-containing TrxR1 is essential for self-sufficiency of cells by control of glucose-derived H2O2
Compensatory changes in Txnrd1−/− and reconstituted TrxR1 variant MEFs. (a) Total GSH contents of the MEF cell lines incubated with or without 250 μM BSO and/or 25 nM selenite for 48 h are shown. (b) Total cellular Trx activities with or without 25 nM selenite supplementation were determined using cell lysates (n=3–4, ±S.E.M.). Significant differences between untreated Txnrd1fl/fl without additional selenite and the other samples are indicated (*P<0.05; n.s., not significant, P>0.05). (c) Total cellular GST activities were determined using the same cell lysates in (b) (n=3–4, ±S.E.M.). Significant differences between untreated Txnrd1fl/fl without additional selenite and the other samples are indicated (*P<0.05; n.s., not significant, P>0.05)
