Figure 3
From: Radiation induces senescence and a bystander effect through metabolic alterations
Radiation induced MCT1 expression through AMPK/glycolysis-dependent pathways in securin-knockdown MDA-MB-231-2A cells. (a) MDA-MB-231-2A cells were exposed to 6 Gy radiation followed by various periods of recovery. The protein expression was examined by western blot analyses. (b) MDA-MB-231-2A cells were pretreated with 5–20 μM compound C (left part) or 5–20 mM DCA (right part) for 2 h and then exposed to 6 Gy radiation. After an 8-h recovery period, the protein expression was examined by western blot analyses. (c) MDA-MB-231-2A cells were pretreated with 10 μM compound C or 10 mM DCA for 2 h and then exposed to 6 Gy radiation. After a 12-h recovery period, MCT1 protein expression was examined by fluorescent microscopy analysis. MCT1 displayed green fluorescence using the anti-rabbit-conjugated FITC antibody. The nuclei were stained with Hoechst 33258
