Figure 5
From: Radiation induces senescence and a bystander effect through metabolic alterations
Radiation induced a bystander effect through AMPK/glycolysis-dependent pathways. (a) MDA-MB-231-2A cells were exposed to 6 Gy radiation followed by various recovery periods. The conditioned media (2A-CM) was collected and used to treat MDA-MB-231 cells. Serum-free and complete medium (10% FBS) were used as negative and positive controls, respectively. After 16 h, cell invasion was examined using the Boyden chamber assay. The numbers of invading cells were observed by microscopy and quantified. **P<0.01 indicates significant differences between 2A-CM- and 10% FBS-treated cells. (b) MDA-MB-231 cells were treated with 2A-CM for various time intervals. The protein expression was examined by western blot analyses. (c and d) MDA-MB-231 cells or HUVECs were pretreated with compound C (c) or DCA (d) for 2 h and then exposed to 2A-CM for 16 h. Cell invasion was examined using the Boyden chamber assay. The number of invading cells was quantified. **P<0.01 indicates significant differences between 2A-CM- and 10% FBS-treated cells. ##P<0.01 indicates significant differences between inhibitor-treated and untreated cells
