Figure 1

Hyperoxia-induced cell death in the OIR retina. (a) Cell death was studied using TUNEL assay on cryostat sections. Numerous apoptotic cells were present in the WT OIR retina. In the A2^−/− OIR retina, fewer TUNEL-positive cells were observed compared with WT OIR retina. (b) Quantification of TUNEL-positive cells showed that the WT OIR retina had increased numbers of apoptotic cells at P8, P9 and P12 (1, 2 and 5 days of hyperoxia, respectively). However, this increase was not observed in the A2^−/− OIR retina. Data are presented as mean±s.d. *WT RA versus WT OIR (P<0.01), ^#WT RA versus WT OIR (P<0.05), ^§A2^−/− OIR versus WT OIR (P<0.01), N varies from four to six. Scale bar=50 μm. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer