Figure 2 | Cell Death & Disease

Figure 2

From: Arginase 2 deficiency reduces hyperoxia-mediated retinal neurodegeneration through the regulation of polyamine metabolism

Figure 2

Expression of arginase 2 (A2) in the OIR retina. (a and b) Immunostaining of retinal cryostat sections on postnatal day 9 showing immunoreactivity for A2 in horizontal cells and in the ONL in the WT RA and hyperoxia-treated WT OIR retina. (c) A2−/− OIR retina used as negative control. Scale bar=50 μm. (d and e) High-magnification confocal images of WT RA and WT OIR retina showing immunolocalization of A2 in the horizontal cells and outer nuclear layer. Scale bar=20 μm. (f and g) High-magnification confocal images of A2 and DAPI showing A2 localization in the area of inner and outer segment development at P9. DAPI shows photoreceptor nuclei. Scale bar=20 μm. (h) Western blot analysis showing the expression of A2 in RA and OIR retinas. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer; OPL, outer plexiform layer; *Area of inner and outer segment development

Back to article page