Figure 3

Analysis of horizontal cells in the WT OIR retina. (a and b) Calbindin immunostaining of horizontal cells on RA and OIR sections. Scale bar=50 μm. (c) Quantification showing a significant decrease in the number of calbindin-positive horizontal cells in WT OIR retina compared with RA controls. *P<0.05, N=5. (d) Colocalization of TUNEL-positive cells with calbindin-positive horizontal cells in WT OIR retina. Scale bar=50 μm. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer