Figure 1 | Cell Death & Disease

Figure 1

From: DNA replication stress in CHK1-depleted tumour cells triggers premature (S-phase) mitosis through inappropriate activation of Aurora kinase B

Figure 1

Induction of Ser10-histone H3 phosphorylation in S-phase CHK1-depleted HCT116 cells during DNA replication stress. (a) HCT116 cells pretreated with control or CHK1 siRNAs were exposed or not exposed to 2 mM thymidine (TdR) for 24 h before collection and analysis for pSer10 histone H3 (top panels, scatter plots) and DNA (bottom panels) content by flow cytometry. Cells with elevated pSer10-histone H3 are in the top quadrants of the scatter plots. S-phase cells showing phosphorylated histone H3 are in the top left quadrant those in G2/M are in the top right. Percentages of cells in each of the gated populations are indicated. (b) Mean values (± S.D.) for three independent experiments as presented above. The effects of nocodazole are also presented. (c) p53−/− HCT116 cells were treated with the CHK1 siRNA in the presence or absence of 2 mM thymidne for 24 h before collection and analysis of pSer10 Histone H3 and DNA content by flow cytometry. (d) Effects of nocodazole treatment on HCT116 or HCT116 p53−/− cells treated as in a, c

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