Figure 3 | Cell Death & Disease

Figure 3

From: DNA replication stress in CHK1-depleted tumour cells triggers premature (S-phase) mitosis through inappropriate activation of Aurora kinase B

Figure 3

CDC45 depletion does not affect induction of pSer10-histone H3 in S-phase HCT116 or HCT116 p53−/− cells depleted of CHK1. (a) Indicated cells pretreated with CHK1 or CHK1 and CDC45 siRNAs were exposed or not exposed to 2 mM thymidine (TdR) for 24 h before collection and analysis for pSer10-histone H3 (top panels, scatter plots) and DNA content (bottom panels) by flow cytometry. (b) Western blot analysis of CDC45 and CHK1 depletion in a representative experiment. β-Actin levels are presented as loading controls. (c) Mean values (± S.D.) for three independent experiments as presented above

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