Figure 5 | Cell Death & Disease

Figure 5

From: DNA replication stress in CHK1-depleted tumour cells triggers premature (S-phase) mitosis through inappropriate activation of Aurora kinase B

Figure 5

Ser10-histone H3 and Aurora kinase B phosphorylation in CHK1-depleted HCT116 p53−/− cells during replication stress are sensitive to Aurora B inhibitors. Western blot analysis of indicated S-phase regulatory and mitotic proteins from extracts of HCT116 p53−/− cells pretreated or not with CHK1 or control siRNAs and exposed or not exposed to thymidine (2 mM, 24 h). Effects of co-treatment with the Aurora A inhibitor MLN8237 (10 nM) or the Aurora B inhibitors AZD1152 (50 nM) and ZM447439 (2 μM) are also presented. Inhibitors were added 1 h before cell collection. β-Actin levels are presented as loading controls

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