Figure 4
Dabrafenib inhibits RIP3 independently of its effect on the B-Raf family members. (a) The B-RafV600E inhibitors GDC-0879 (GDC), vemurafenib (VEM) and dabrafenib (DAB) inhibited the phosphorylation of MEK and ERK in B-RafV600E-expressed A375 cells and HT29 cells. con, control. The data were representative of three independent experiments. (b) The cell viability of the cells exposed to TSZ in the presence or absence of the indicated B-RafV600E inhibitors for 24 h was examined. All the B-RafV600E inhibitors were used at 1 μM in A375 cells while at 10 μM in both shRIP3 and shNC N cells. (c) MLKL was silenced with the specific siRNA in HT29 cells. The protein levels were examined by western blotting. The cell viability of the cells exposed to the indicated combinations for 24 h was examined. Dabrafenib was used at 10 μM. *P=0.00002; **P=0.0002. (d) B-Raf was silenced with the specific siRNA in HT29 cells. The protein levels were examined by western blotting (left). The cell viability of HT29 cells exposed to the indicated combinations for 24 h was examined
