Figure 5
Protection of dabrafenib from acetaminophen-induced cell viability reduction in human hepatocytes. (a) Human liver QSG-7701 and HL-7702 cells were treated with 20 mM acetaminophen in the presence or absence of 10 μM dabrafenib for the indicated times and the cell viability was examined. Insets, the levels of RIP1 and RIP3 proteins in the cells were detected by western blotting. (b) The cells exposed to 20 mM acetaminophen for the indicated times in the presence or absence of 10 μM dabrafenib were observed under an inverted microscope at × 20 magnification. Scale bar, 50 μm. (c) The effects of dabrafenib and necrostatin-1 on QSG-7701 and HL-7702 cells treated with 20 mM acetaminophen for 10 or 21 h were examined. (d and e) RIP3 (d) or RIP1 (e) was silenced with the specific siRNA in QSG-7701 cells and HL-7702 cells. The protein levels were examined by western blotting. The cell viability of the indicated cells exposed to 20 mM acetaminophen at the indicated times was examined. *P=0.004; **P=0.005; ***P=0.03; ****P=0.05. All the image data were representative of three independent experiments
