Figure 2

CT45A1 enhances tumorigenesis in breast cancer in situ xenografted mice. After MCF7-V or MCF7-CT45A1 cells were cultured with SF-EFB for 5 days, the cells were harvested and injected into the breast cushion of NOD-SCID mice (n=6), and the tumor volume was measured every other days (a), the excised tumors were photographed (b), and weighted (c). The tumor invasion in the breast area of the mice was photographed (d), the green line marked the breast in situ xenografted area, and the red line marked the rib bone area; tumor cell invasion to the vicinity of the rib bone was noted in MCF7-CT45A1 cell-xenografted mice (d, right panel), but absent in CT45A1-negative MCF7-V cell-xenografted mice (d, left panel). The experiments were repeated twice. Samples were compared using one-way ANOVA, and error bars of the data indicated mean±S.E.M., *P<0.05, **P<0.01, as compared with MCF7-V cells. The lung tissues in MCF7-V and MCF7-CT45A1 cell-xenografted mice were excised and sectioned. The tissue slides were stained with H&E method and imaged at × 100 (e, left two panels) and × 400 (e, right two panels); (f) in addition, the slides were triply stained with CT45A1-specific antibody (green color), breast cancer cell marker CD44 antibody (red color), and DAPI (blue color), and photographed with confocal microscopy at × 1000