Figure 6
MPTP intoxication of adult mBACtgDyrk1a mice. (a) Schedule of MPTP intoxication employed in experiments in (b and c). (b) Dual immunohistochemistry for TH and active Casp9 (A-Casp9) in the SN pars compacta of a wild-type (WT) animal 4 days after MPTP intoxication, showing a pyknotic cell (arrowhead) and an active Casp9+ (black) cell (arrow) expressing TH (brown), and the total numbers of active Casp9+ cells quantified in the SN pars compacta of WT and mBACtgDyrk1a (BACD1a) animals injected with saline (Sal.) or MPTP. (c) Representative images showing TH+ cells in the SN pars compacta of saline- or MPTP-treated WT and BACD1a animals 21 days after MPTP intoxication, and the quantification of the total TH+ cells in this region for the four experimental groups. Histogram values are the mean±S.E.M. N, not significant (P>0.05); *P≤0.05; **P≤0.01; ****P≤0.0001 (n=3; p-ANOVA=0.001 in (b) and n=5; p-ANOVA=0.01 in (c)). Bar=100 μm (b and c)
