Figure 2

GC-MSCs-primed neutrophils prompt gastric cancer cell migration and endothelial cell tube formation. Neutrophils were pretreated with GC-MSCs conditioned medium for 12 h, and then cocultured with 2 × 10⁴ SGC-7901 cells in the upper compartment of the chamber. After incubation for 12 h, the migrated cells on the lower surface of the membrane were stained with crystal violet after fixation and then counted under a light microscope. For the transwell coculture, SGC-7901 cells were seeded in the upper chamber and neutrophils in the lower chamber (a); HUVECs were seeded at 2 × 10⁴ cells/well and incubated with conditioned media from GC-MSCs-primed neutrophils for 10 h. The formation of tube-like structure by HUVECs was observed under a phase-contrast microscope and photographed at 100 × magnification (b). Scale bar=200 μm. **P<0.01