Figure 4

Blocking STAT3–ERK1/2 signaling cascade inhibits GC-MSCs-induced pro-migratory and pro-angiogenic roles in neutrophils. Neutrophils were stimulated with GC-MSCs CM in the presence or absence of U0126 (10 μM) or WP1066 (10 μM), and then the cells were collected. Neutrophils were seeded with 2 × 10⁴ SGC-7901 cells in the upper compartment of the chamber for coculture. After incubation for 12 h, the migrated cells on the lower surface of the membrane were stained with crystal violet after fixation and then counted (a, c); HUVECs were seeded at 2 × 10⁴ cells/well with conditioned media from neutrophils for 10 h. The formation of tube-like structure by HUVECs were observed under a phase-contrast microscope and photographed at 100 × magnification (b, d). Scale bar=200 μm. *P<0.05, **P<0.01