Table 1 Primer sequences of target genes

From: The IL-6–STAT3 axis mediates a reciprocal crosstalk between cancer-derived mesenchymal stem cells and neutrophils to synergistically prompt gastric cancer progression

Genes

Primer sequence (5′–3′)

Amplicon size (bp)

Annealing temp (°C)

Mcl-1

For: ACGGCCTTCCAAGGCAT

103

63

 

Rev: TTGTTACGCCGTCGCTGA

  

Fas

For: TCGGAGGATTGCTCAACAAC

183

63

 

Rev: ATGATGCAGGCCTTCCAAGT

  

IL-8

For: GCTCTGTGTGAAGGTGCAGTTT

144

62

 

Rev: TTCTGTGTTGGCGCAGTGT

  

OSM

For: CACAGAGGACGCTGCTCAGT

143

60

 

Rev: GCTGGTGTCCTGCATGAGA

  

CCL2

For: GAACCGAGAGGCTGAGACTA

151

52

 

Rev: GCCTCTGCACTGAGATCTTC

  

TNFα

For: CCGAGTGACAAGCCTGTAGC

493

57

 

Rev: AGGAGGTTGACCTTGGTCTG

  

IL-6

For: TACATCCTCGACGGCATCTC

252

61

 

Rev: AGCTCTGGCTTGTTCCTCAC

  

α-SMA

For: CTGACTGAGCGTGGCTATTC

452

58

 

Rev: CCACCGATCCAGACAGAGTA

  

FAP

For: ATAGCAGTGGCTCCAGTCTC

278

59

 

Rev: GATAAGCCGTGGTTCTGGTC

  

β-actin

For: CACGAAACTACCTTCAACTCC

265

56

 

Rev: CATACTCCTGCTTGCTGATC

  
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