Figure 1 | Cell Death & Disease

Figure 1

From: Retinal pigment epithelial cells undergoing mitotic catastrophe are vulnerable to autophagy inhibition

Figure 1

Rotenone induces mitotic catastrophe in ARPE-19 RPE cells. R, rotenone. (a) Viability assay, as determined by trypan blue exclusion. The values are expressed as the mean±S.D. **P<0.01 to the control. (b) Giemsa staining showing that ARPE-19 cells treated with rotenone have multiple nuclei. The numbers indicate the percentage of cells with multiple nuclei. The values are expressed as the mean±S.D. (c) Flow cytometry analysis indicating that most rotenone-treated ARPE-19 cells were tetraploid by 48 h, as shown by a 4C DNA peak. Representative data are presented. (d) Transmission electron microscopy demonstrates the presence of micronuclei (arrows) in the rotenone-treated, multinucleated RPE cells. N, nucleus

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