Figure 2 | Cell Death & Disease

Figure 2

From: Retinal pigment epithelial cells undergoing mitotic catastrophe are vulnerable to autophagy inhibition

Figure 2

Increased autophagy occurs in the RPE-MC cells. C, control. (a) Western blotting analysis demonstrating an increase in the LC3-II levels in the rotenone-treated ARPE-19 cells. (b) Immunoprecipitated beclin-1 complex from the ARPE-19 cells. The interaction between beclin-1 and VPS-34 was increased, and the interaction between beclin-1 and Bcl-2 was decreased in the rotenone-treated cells compared with the control cells. (c) Flow cytometry analysis demonstrating that rotenone increased the development of acidic vesicular organelles. Representative data are presented. (d) Transmission electron microscopy showing that rotenone increased the number of autophagosomes or autophagolysosomes (arrows) in the RPE-MC cells

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