Figure 1 | Cell Death & Disease

Figure 1

From: EGF-induced adipose tissue mesothelial cells undergo functional vascular smooth muscle differentiation

Figure 1

Efficient recovery of ATMCs by gentle trypsinization of the uterine AT. (a) Aspect of uterine AT depots before (left) and after (middle) quirurgical separation from uterine cords. Right photograph shows representative aspect of cells isolated through gentle trypsinization of AT. Trypsin typically detached small sheets (spot) of flattened epitheloid cells that adopted ‘grape-like’ structure after full retraction. (b) Trypsin isolated cells widely immunoexpressed epithelial (β-catenin, E-cadherin and ZO-1) and CD54. They also immunoexpressed more heterogeneously Sca1 and lacked expression of the VSMCs marker smoothelin-B (Smtn-B). (c) Flow cytometry analysis expression results of several surface markers in trypsin isolated cells (n=5). (d) Trypsin isolated cells cultured for 5 days in BMe exhibited cobblestone morphologies (phase contrast) and immunoexpressed WT1, CD54, ZO-1, E-cadherin and β-catenin. (b and d) Nuclei are counterstained in blue with Hoechst 33342

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