Figure 4

Western blotting analysis of phenotypic changes undertaken by ATMCs upon sequential subculture into BMe+50EGF. Expression of markers expressed in the mesothelial (a), SM (b) and cardiac (c) lineages as well as the phosphorylation pattern of the EGF receptor, Akt, ERK1/2 and Smad2 (d) were evaluated in freshly isolated ATMCs (0 h) and ATMCs after 3, 5, 10 and 15 days of culture in BMe+50EGF. FVO, are proteins from mouse fetal (E17) visceral organs (heart, lung and peritoneal organs). FH, are proteins from mouse fetal (E17) hearts