Figure 6 | Cell Death & Disease

Figure 6

From: EGF-induced adipose tissue mesothelial cells undergo functional vascular smooth muscle differentiation

Figure 6

Generation of spreading ATMC-derived VSM-like cell spheroids for their use in contraction studies. (a) Schematic representation of steps performed to generate ATMC-derived VSM-like cell spheroids. Right, phase contrast pictures of spheroid taken after 0 and 24 h of their transfer onto plastic adherent surface into BMe+50EGF. Scale bar is 200 μm. (b) Immunofluorescence characterization of SMCs markers (SM22α and αSMA) expression in spheroids fixed after 24 h of spreading onto plastic surface

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