Figure 3
From: Inhibition of apoptotic Bax translocation to the mitochondria is a central function of parkin
The scale of parkin-mediated mitophagy is affected by cell type. Dopaminergic MES cells (a), and HEK cells (b), each with or without stable expression of parkin, were treated with either DMSO or 20 μM CCCP for 24 h in triplicate. Whole-cell lysates were probed for outer (VDAC and TOM20) and inner (Complex II and V) mitochondrial proteins to assess mitophagy. For quantification, band intensities of proteins were normalized to vehicle (DMSO). (c) MES, (d) HEK and (e) CHO cells, with or without stable expression of parkin, were treated with either DMSO or 20 μM CCCP for 4 or 24 h, and isolated mitochondrial fractions were probed for parkin translocation and for outer and inner mitochondrial proteins to assess mitophagy. For quantification, band intensities of proteins from mitochondrial fractions were normalized to vehicle (DMSO). All bars represent mean±S.D., n=3. Asterisks indicate significant differences compared with control (P<0.05)
