Figure 8
From: Inhibition of apoptotic Bax translocation to the mitochondria is a central function of parkin
Disease-associated mutation of parkin is unable to regulate Bax translocation to the mitochondria. (a) Lysates from CHO cells stably expressing WT parkin or mutant R275W-parkin. (b) CHO cells expressing WT or R275W Parkin were transiently transfected with WT GFP-Bax and the mitochondrial marker mito-mCherry. Representative images of fixed cells showing localization of GFP-Bax after treatment with DMSO or 1 μM staurosporine (Sts) for 5 h. Scale bar, 20 μm. (c) Quantification of b. Cells were scored as described in Figure 5. Forty-six to 64 cells were counted per condition. Data plotted from three independent experiments. (d) Percentage of pooled mitochondrial fractions (predominantly mitochondrial+completely mitochondrial) plotted from b. All bars represent mean±S.D., n=3. Asterisk indicates significant difference when compared with WT (P<0.05).
