Figure 3
miR-203 represses chicken primary myoblast proliferation and induces cell cycle arrest. (a) miR-203 expression determined by qPCR in myoblasts transfected with miR-203 mimic or control duplexes. (b) Cell growth was measured following the transfection of miR-203 mimic or control duplexes into primary myoblasts in GM at 24, 48, 72 and 96 h. (c) The number of EdU-stained cells is significantly reduced after transfection with the miR-203 mimic. (d) The proliferation rate of myoblasts transfected with miR-203 is significantly reduced compared with that of control myoblasts. (e) Primary myoblasts were collected for cell cycle analysis 48 h after transfection. Propidium iodide staining for DNA content and a FACSAriaII flow cytometer were used to determine the percentage of cells in G0/G1, S and G2/M. (f) miR-203 expression determined by qPCR in myoblasts transfected with anti-miR-203 or anti-NC. (g) Cell growth was measured following the transfection of anti-miR-203 or anti-NC into primary myoblasts in GM at 24, 48, 72 and 96 h. (h) Primary myoblasts were collected for cell cycle analysis 48 h after transfection. Propidium iodide staining for DNA content and a FACSAriaII flow cytometer were used to determine the percentage of cells in G0/G1, S and G2/M. Results are expressed as the mean±S.E.M. of three replicates. (*P<0.05; **P<0.01)
