Figure 6

c-JUN promotes myoblast proliferation. (a) Overexpression of c-JUN via pcDNA3.1-c-JUN transfection increases the c-JUN protein level. (b) Cell growth is significantly increased after pcDNA3.1-c-JUN transfection compared with that in control cells. (c) Overexpression of c-JUN results in an increased cell population in the S phase and a decreased cell population in the G0/G1 phase. (d) Knockdown of c-JUN via si-c-JUN transfection reduces the c-JUN protein level. (e) Cell growth is significantly reduced after si-c-JUN transfection compared with that in control cells. (f) Knockdown of c-JUN results in an increased cell population in the G0/G1 and G2/M phases, and a decreased cell population in the S phase. All of the results are expressed as the mean±S.E.M. of three replicates. *P<0.05; **P<0.01; ***P<0.001