Figure 4
From: Bone marrow-derived clonal mesenchymal stem cells inhibit ovalbumin-induced atopic dermatitis
Suppression of isotype class switching and B-cell differentiation by cMSCs. (a) RNA was isolated from stimulated B cells. ɛ-GLT and ɛ-PST mRNA levels were significantly reduced in LPS/IL-4-stimulated B cells by cMSCs, whereas both transcripts were weakly decreased by cMSCs under anti-CD40/IL-4 stimulation. (b) Other transcription factors for B-cell differentiation were analyzed by real-time PCR. (c) The cMSCs inhibited BLIMP-1 and AID protein expression in B cells cultured for 4 days. Similar results were obtained in two to three independent experiments. *P<0.001
