Figure 6
From: Improving the therapeutic potential of endostatin by fusing it with the BAX BH3 death domain
Interaction of ES, ES-BAX, and ES-BAK with members of the BCL-2 family. Whole cell lysates of 107 endothelial cells (HUV-EC-C) were incubated in the absence or presence of 100 μg/ml ES, ES-BAX, or ES-BAK. The ES, ES-BAX, or ES-BAK complexes bound to cellular proteins (BCL-2, BCL-XL, BAX, or BAK) were incubated with the antibodies indicated for co-immunoprecipitation, captured on Protein G agarose beads, and subsequently subjected to SDS-PAGE. The complexes were detected by immunoblotting with the indicated antibodies. Similar blots were probed with the same antibodies that were used for co-immunoprecipitation, to ensure that equivalent amounts of proteins were present in each reaction. Cell lysates were applied at equivalent volumes. Non-related monoclonal antibodies were used to control co-immunoprecipitation
