Figure 2 | Cell Death & Disease

Figure 2

From: S100A2 is a BRCA1/p63 coregulated tumour suppressor gene with roles in the regulation of mutant p53 stability

Figure 2

(ai) Western blot analysis of BRCA1 mutant HCC 1937 cells stably transfected with either EV or wild-type BRCA1 (BR) and transiently transfected with siRNA targeting ΔNp63 (DNp63si) or scrambled control (Scr). Blots were probed with p63, S100A2 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (aii) Real-time polymerase chain reaction (PCR) analysis of the same samples as (i) with specific primers for S100A2 and β-tubulin as a housekeeper. (bi) Western blot analysis of BRCA1 low MDA468 cells stably transfected with either EV or wBR and transiently transfected with siRNA targeting ΔNp63 (DNp63si) or Scr. Blots were probed with p63, S100A2 and GAPDH as a loading control. (bii) Real-time PCR analysis of the same samples as (i) with specific primers for S100A2 and β-tubulin as a housekeeper. (ci) Western blot analysis of MCF-7 cells transiently transfected with siRNA targeting ΔNp63 (DNp63si), TAp63 (TAp63si), p53 (p53si) or Scr. Blots were probed with S100A2 and GAPDH as a loading control. (cii) Western blot analysis of MCF-7 cells transiently transfected with siRNA targeting p63γ (p63γsi), p63α and β (p63α/β si), all p63 isoforms (pan-p63), AP2α (AP2αsi), AP2γ (AP2γsi) and Scr. Blots were probed with S100A2 and GAPDH as a loading control. (d) Western blot analysis of HCC BR cells or HCC 1937 cells transiently transfected with EV, ΔNp63α, ΔNp63γ, TAp63α or TAp63γ. Blots were probed for S100A2, p63 and GAPDH as a loading control

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