Figure 2

Astroglial PARP1 expression in the hippocampus following SE. (a) Astroglial PARP1 degradation in the molecular layer of the dentate gyrus following SE. In the molecular layer of the dentate gyrus, PARP1 expression is detected in astrocytes. Following SE, PARP1 expression in astrocytes is undetectable in this region accompanied by downregulation of GFAP expression. Bar=12.5 μm. (b) Astroglial death induced by SE. Following SE, both GFAP and GS expression are reduced in the molecular layer of the dentate gyrus. M, molecular layer; DG, dentate granule cell layer; H, hilus. Bar=50 μm. In addition, TUNEL-positive astrocytes show downregulation of GFAP expression. Bar=12.5 μm. (c) Astroglial PARP1 induction in the stratum radiatum of the CA1 region following SE. In non-SE animals, few astrocytes show PARP1 immunoreactivity in the CA1 region. Following SE, reactive astrocytes contain PARP1 immunoreactivity. Bar=12.5 μm. (d) The changes in the number of total astrocytes and PARP1-positive astrocytes in the hippocampus following SE. In the molecular layer of the dentate gyrus (DG), the fraction of PARP1-positive astrocytes in total astrocytes is reduced following SE. In the CA1 and CA3 regions, the numbers of total astrocytes and the fraction of PARP1-positive astrocytes in total astrocytes are gradually increased following SE (means±S.D., n=5, respectively). *P<0.05 versus non-SE animals (one-way ANOVA test)