Figure 3
From: Targeting Btk/Etk of prostate cancer cells by a novel dual inhibitor
CTN06 is a potent Etk and Btk dual inhibitor. (a) Chemical structure of CTN06. (b) The potency of CTN06 to Btk, Etk, Src and Mer was measured using TLC to identify 33P-phosphorylated peptide substrate. Purified TKs (20 nM), CTN06 (0–10 μM) and the peptide substrate (YIYGSFK) were incubated with 33P-ATP in a kinase reaction. The resulting product was analyzed on a TLC plate. (c) The intensity of the radioactive spot was measured using densitometer. (d) Summarization of the kinase inhibition profile of CTN06. Columns, mean; bars, standard deviation, n=3. Molecular docking of CTN06 to Btk. (e) Surface view of Btk docked to CTN06 after 20 ns of MD relaxation. Brown: helix C (439–452); green: activation loop helix 1 (AH1) (541–546) and activation loop helix 2 (AH2) (548–553); yellow: DFG motif (538–540); blue: Thr474; red: Cys480. (f) Cartoon representation showing predicted interactions of Btk with CTN06. Thr474, Leu408, Cys480 and Asp538 side chains are shown as sticks and colored based on residue type (red: acidic, green: polar, yellow: non-polar). DFG motif is shown in red. Figures were generated using PyMol
