Figure 4
From: Amyloid-β induces NLRP1-dependent neuronal pyroptosis in models of Alzheimer’s disease
NLRP1 silencing rescued early cognitive deficits in APPswe/PS1dE9 mice. Results from 6.5-month-old wild-type (WT) and APP/PS1 mice after 6 weeks of treatment with NLRP1 siRNA or control siRNA using a miniosmotic pump. During the last 6 days of the treatment, Morris water maze test was conducted to assess the cognitive functions of mice. The Morris water maze test consists of a hidden-platform training last for 5 consecutive days, plus a probe trial conducted at 24 h after the hidden-platform trial. (a and b) Time to platform (escape latency) and distance to platform of each group in the hidden-platform training. Data were analyzed by two-way repeated-measures ANOVA. #P<0.05 versus WT mice treated with control siRNA. §P<0.05 versus APP/PS1 mice treated with control siRNA. (c) Data from probe trials are presented as percentage of time spent in the target quadrant (Q1) versus the averaged time spent in all other (a.o.) quadrants. Q1, quadrant where platform was located on days 1–5. Data were analyzed by independent sample t-test. *P<0.05 versus averaged time spent in a.o. quadrants. (d and e) To exclude differences in motor performance between groups, swimming speed and path length were recorded and analyzed by a computer-controlled tracking system. Statistical analysis did not reveal any significant differences between groups. Each value represents the mean±S.E.M. from 18 male mice per group
