Figure 1
From: Targeting the actin cytoskeleton: selective antitumor action via trapping PKCɛ
Chondramide A (ChA) inhibits actin dynamics and leads to agglomeration of actin. (a) Molecular structure of ChA. (b) MCF7 cells were transfected with mGFP-β-actin and treated with 300 nM ChA for 30 min. The mobile actin fraction was quantified by FRAP analysis. (Upper panel) White arrows indicate the photobleached area in representative images (Ctrl, untreated cells; Ch300, cells treated with 300 nM ChA). (Lower panel) Values represent the % fluorescence recovery over time of mGFP-β-actin after photobleach. (c) Time course of actin hyperpolymerization of MCF7 cells expressing mGFP-β-actin and treated with 300 nM ChA (Ch300) for 0.5, 6 and 24 h. Scale bar indicates 10 μm. Each experiment was independently performed three times
