Figure 1
From: Radiosensitization by a novel Bcl-2 and Bcl-XL inhibitor S44563 in small-cell lung cancer
Effect of S44563 on cell viability and cell survival. (a) Chemical structure of S44563. (b) Inhibition of the interaction between Bcl-2 or Bcl-XL and fluorescent Puma BH3 peptide measured by the decrease of fluorescence polarization as a function of S44563 concentrations. Three independent experiments are presented. FP data are presented in millipolarization units (mP). Each experiment was performed in triplicates (mean +/−S.E.M., three experiments). (c) Bcl-2/Bax complex disruption by S44563 measured by co-immunoprecipitation assays. Cell lysates were subjected to immunoprecipitation with an anti-Bcl-2 antibody and immunoprecipitates and lysates were analyzed by immunoblot with an anti-Bax antibody. (d) Caspase 3 activation by S44563 in H146 cell line. Caspase 3 enzymatic activity is presented as Relative Fluorescent Unit (RFU) per minute and per mg of protein (mean +/−S.E.M., three experiments). (e) Inhibition of SCLC cell proliferation by S44563. The cells were seeded 24 h before S44563 was administered with various concentrations from 10 nmol/l to 10 μmol/l for 72 h. The number of viable cells was determined by using WST-1 assay according to the manufactrurer’s instructions (Roche). Absorbance values were normalized to the values obtained from untreated cells to determine survival rates. Each assay was performed in triplicate (points, mean; bars, standard error deviation, three experiments)
