Figure 1

MEIS2 is essential for neuroblastoma cell survival. (a) Immunoblotting of MEIS2 in a panel of 13 human neuroblastoma cell lines. β-Actin levels are shown as a loading control. (b and c) qRT-PCR (b) and immunoblot (c) analyses of MEIS2 expression in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. Error bars (b) represent S.D. (n=3). GAPDH (c) serves as a loading control. (d) Micrographs of BE(2)-C cells infected for 3 days with lentiviruses expressing either shGFP or shMEIS2. (e) Trypan blue exclusion assay of viable BE(2)-C cells infected for 4 days with lentiviruses expressing either shGFP or shMEIS2