Figure 2 | Cell Death & Disease

Figure 2

From: MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression

Figure 2

MEIS2 depletion induces M-phase arrest and mitotic catastrophe. (a and b) Cell-cycle analysis (a) and quantification (b) showing that MEIS2 depletion induces M-phase arrest in BE(2)-C and SK-N-F1 cells. Error bars (b) represent S.D. (n=3). (c and d) Immunofluorescence staining (c) and quantification (d) of phospho-histone H3+ M-phase BE(2)-C cells following infection with lentiviruses expressing either shGFP or shMEIS2-43. Error bars (d) represent S.D. Approximately 400–1000 cells (DAPI positive) were counted from at least 10 random × 630 fields. (e) Hoechst 33342 staining of nuclei showing chromatin condensation and micronucleation (arrows) in BE(2)-C cells following infection with lentiviruses expressing shMEIS2-43 or shMEIS2-44. Data (b and d) were analyzed with two-tailed Student’s t-test with the P-values indicated

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