Figure 3 | Cell Death & Disease

Figure 3

From: MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression

Figure 3

MEIS2 depletion induces multipolar mitotic spindles and centrosome amplification. (a–c) Immunofluorescence staining for M-phase BE(2)-C cells (phospho-H3+) and mitotic spindles (α-tubulin) following infection with lentiviruses expressing shGFP (a), shMEIS2-43 (b), or shMEIS2-44 (c). (d) Quantification of M-phase BE(2)-C cells (phospho-H3+) with multipolar spindles. (e–g) Immunofluorescence staining for M-phase BE(2)-C cells (phospho-H3+) and centrosomes (γ-tubulin) following infection with lentiviruses expressing shGFP (e), shMEIS2-43 (f), or shMEIS2-44 (g). (h) Quantification of M-phase BE(2)-C cells (phospho-H3+) with more than two centrosomes. Error bars (d and h) represent S.D. Approximately 400–1000 cells (DAPI positive) were counted from at least 10 random × 630 fields. Data (d and h) were analyzed with two-tailed Student’s t-test with the P-values indicated. Pro, prophase; Meta, metaphase; Ana, anaphase; and Telo, telophase

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