Figure 6 | Cell Death & Disease

Figure 6

From: MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression

Figure 6

MEIS2 enhances the proliferation and tumorigenicity of neuroblastoma cells. (a) qRT-PCR analysis of mRNA expression of RBBP4, BMYB, FOXM1 and their target genes in BE(2)-C cells transfected with plasmids expressing individual MEIS2 isoforms. Error bars, S.D. (n=3). (b) qRT-PCR analysis of mRNA expression of FOXM1 and its key target genes in BE(2)-C cells co-transfected with an MEIS2d-expessing plasmid along with constructs expressing shGFP, shFOXM1-44, or shFOXM1-84. Error bars, S.D. (n=3). **P<0.01. (c) Immunoblotting of MEIS2d in BE(2)-C_tetoff_MEIS2d cells with or without doxycycline (Doxy). α-Tubulin levels are shown as a loading control. (d and e) Growth (d) and soft agar (e) assays of BE(2)-C_tetoff_MEIS2d in the presence or absence of Doxy. (f) Xenograft assay of BE(2)-C_tetoff_GFP and BE(2)-C_tetoff_MEIS2d cells. Tumor weight was analyzed by scatter plot with horizontal lines indicating the mean. Data (b, d, e, and f) were analyzed with two-tailed Student’s t-test with the P-values indicated

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