Figure 1
From: Glucose metabolism is inhibited by caspases upon the induction of apoptosis
The ATP decrease during apoptosis is caspase dependent. (a) HeLa cells were plated in control media and were then either deprived of glucose for 16 h and/or incubated in the presence of oligomycin D (oligo D, 10 ng/ml) 1 h before ATP measurement. (b) HeLa cells were plated in 96-well plates and treated as indicated with staurosporine with or without qVD-OPH (20 μM). ATP was then measured, and the results are expressed in relative luminescence units. (c) HeLa cells were treated with 1 μM actinomycin D (Act D) or with 100 ng of recombinant TRAIL as indicated and analyzed in a. Tables in b and c represent the % of annexin V/propidium iodide (AV+/PI+/−) cells in indicated conditions. (d) HeLa cells were treated as in b and DEVDase activity was measured with or without qVD-OPH (20 μM). **P<0.005
