Figure 6 | Cell Death & Disease

Figure 6

From: UDP glucuronosyltransferase 1A expression levels determine the response of colorectal cancer cells to the heat shock protein 90 inhibitor ganetespib

Figure 6

UDP glucuronosyl conjugation of ganetespib. (a and b) HCT116, SW480, SW1463, and HT29 cells were treated with 1 μM ganetespib. Cell lysates and culture media were collected at 5, 15, 30, 60, and 480 min. The total concentration of ganetespib and its glucuronidated metabolites in the lysates (a) and the supernatant media (b) were determined by LC-MS/MS. (c and d) HCT116 and HT29 cells were treated with 1 μM NVP-AUY922. Cell lysates and culture media were collected at 5, 15, 30, 60, and 480 min. The total concentration of NVP-AUY922 and its glucuronidated metabolites in the lysates and the supernatant media were determined by LC-MS/MS. The levels of glucuronidated NVP-AUY922 are given as NVP-AUY922 glucuronide peak area ratios

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