Figure 2

Kinetics of ABT737-induced loss of mitochondrial membrane potential (MMP) promotes cell death in HaCaT and A5RT3 cells, but not in PKs or MET cells. (a and b) PKs (donors E and L), HaCaT, HaCaT-derived A5RT3 and MET1 or MET4 cells were either stimulated with 10 μM ABT737, the respective Enantiomer or with DMSO for 24 or 48 h (control). The loss of MMP was investigated by FACS using staining with TMRE as described in the Materials and Methods. Quantitative summary of three independent experiments is shown in (b). (c and d) HaCaT and A5-RT3 cells were stimulated with ABT737 (10 μM), inactive Enantiomer or DMSO (control) alone for the indicated times. Loss of MMP was analysed as described for (a). The quantitative summary of TMRE-positive cells of two independent experiments with stimulation with 10 μM of ABT737 is shown in (d). (e and f) Apoptotic cell death of the indicated cells was analysed by hypodiploidy analysis after stimulation with ABT737 (10 μM), Enantiomer (10 μM) or DMSO (control) for 24 and 48 h and the quantification of two independent experiments is depicted in (f)