Figure 4 | Cell Death & Disease

Figure 4

From: Viability and stress protection of chronic lymphoid leukemia cells involves overactivation of mitochondrial phosphoSTAT3Ser727

Figure 4

pSTATSer727 associates with complex I of the mitochondria respiratory chain. (a) Mitochondria (Mt) were isolated from CLL-BC (whole cells, WC) by magnetic immunolabeling. Extracts were separated by SDS-PAGE and blots were probed with the indicated antibodies. (b) Antibody to complex I (CI) of the ETC or a nonspecific isotype-matched IgG (ctl) were incubated with CLL-BC or N-BC total cell extracts (TEs), as indicated. Immunoprecipitates (IPs) were resolved on SDS-PAGE and probed for the indicated proteins. 1 : 50 of TE was loaded on the gel. α and β indicate STAT3α and STATβ isoforms, respectively

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