Figure 2 | Cell Death & Disease

Figure 2

From: Mycobacterium tuberculosis ESAT-6 is a leukocidin causing Ca2+ influx, necrosis and neutrophil extracellular trap formation

Figure 2

ESAT-6 causes selective necrosis of phosphatidylserine externalised neutrophils. (a) Addition of ESAT-6 causes an increase in the proportion of propidium iodide positive cells as assessed by flow-cytometry. Control protein is recombinant Rv0435c. Ionomycin is a known inducer of neutrophil necrosis. Data normalised to vehicle in each experiment. Accumulated data N>4 for each condition (three repeats per experiment). *P<0.05 using Student’s unpaired t test compared to control protein. (b) The increase in necrosis caused by ESAT-6 (20 μg/ml) occurred preferentially in PS externalised cells (Annexin V-FITC, AnV+ve). N=4 (in triplicate). *P<0.05 using unpaired Student’s t test. Error bars are S.E.M.

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