Figure 1 | Cell Death & Disease

Figure 1

From: AML cells carrying NPM1 mutation are resistant to nucleophosmin displacement from nucleoli caused by the G-quadruplex ligand TmPyP4

Figure 1

(a) Viability of OCI-AML2 and OCI-AML3 cells, untreated (CTR) and treated with TmPyP4 50 or 100 μM at the indicated time points (hours). (b) Percentage of cell death for OCI-AML2 and OCI-AML3 cells after treatment, as described above. (c) Western blot analysis of OCI-AML2 and OCI-AML3 cells, using antibodies against nucleophosmin (NPM1), p53 and β-actin as a loading control. The corresponding molecular weights are indicated on the left. (d) Western blot analysis of OCI-AML2 and OCI-AML3 cells treated with 100 μM TmPyP4 for the indicated time points (hours) using antibodies against p53 and β-actin as a loading control. (e) Real-time PCR of RNA extracted from OCI-AML2 and OCI-AML3 cells, untreated (CTR) or treated with 100 μM TmPyP4 for the indicated time points (hours) using primers for human p21

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