Figure 3
Psn overexpression induces a JNK pathway-independent apoptosis. (a, a') Anti-MMP1 staining in vg-GAL4/+ (a) and vg-GAL4, UAS-Psn, UAS-Psn/+ (a') third-instar wing imaginal discs. (b–c') Anti-β-Galactosidase staining to detect the expression of msn- (b) or puc-lacZ (c, d) reporters in vg-GAL4/+; msn06946/+ (b), vg-GAL4, UAS-Psn, UAS-Psn/+; msn06946/+ (b'), vg-GAL4/+; pucE69/+ (c) and vg-GAL4, UAS-Psn, UAS-Psn/+; pucE69/+ (c') third-instar wing imaginal discs. Note MMP1 staining (a) and msn-lacZ expression (b) in the notum (white arrowheads) are constitutive and independent from Psn overexpression. Note that puc-lacZ expression at the tip of the notum reflects the JNK pathway activation required for thorax closure (c, white arrow). (d, d') Colocalization (white) between anti-β-Galactosidase staining (green) to detect puc expression and either TUNEL labeling (d, red) to detect apoptosis or anti-PH3 (d', red) to detect mitotic cells in vg-GAL4, UAS-Psn, UAS-Psn/+; pucE69/+ third-instar larvae wing imaginal discs. (e–e''') TUNEL staining in vg-GAL4, UAS-Psn, UAS-Psn/+ (e), UAS-bskDN/+ vg-GAL4, UAS-Psn, UAS-Psn/+ (e'), vg-GAL4, UAS-Psn, UAS-Psn/+; UAS-hep-RNAi/+ (e') and in vg-GAL4, UAS-Psn, UAS-Psn/UAS-cdk5-RNAi (e''') third-instar wing imaginal discs
