Figure 1

WA promotes apoptosis in cancer cells lacking p53. (a) H1299 and HCT116^TP53−/− cells were treated with WA for 48 h and stained with crystal violet for clonogenic survival determination. (b) Growth inhibition correlated with caspase activation by WA. (c) Caspase inhibitor zVAD-FMK protects H1299 and HCT116^TP53−/− from growth inhibition by WA. (d) WA stimulates accumulation of H1299 in subG1 fraction, which is reversed by zVAD-FMK pretreatment. (e and f) Immunoblots presenting induction of TAp73 in HCT116^TP53−/− and in H1299 cells, which coincides with the induction of apoptotic PUMA and with the PARP cleavage. (g) HCT116^TP53−/− cells after treatment with WA or H₂O₂ (100 μM) for 3 h ( × 100 magnification) (n=3). (h) Immunoblot of WA-treated NHDF presenting TAp73, TAp63 and BAX protein levels. (i) WST-1 assay performed in MEFs wt TAp73 treated with WA